Mike's NMR Page

Group Wisdom

NMR assignment sequences/processing

Relaxation Stuff

Varian vnmr faq's

Example NMRPipe scripts for Varian

NMRview stuff

non-linear sampling 

barmy
fid

Info on linux clusters

Mike's PubMed Publications

Mike's NMRPipe tutorial

Scheduling jobs over muliple cpu's

NMR flash animations 

Some useful scripts

Protein Expression protocols:

Minimal Media

Notes on expression in E.coli (powerpoint presentation)

NMR Manager:
Mike Osborne

Back to mainpage















Most common commands


















gg Mikey's NMR Page         uni


Basic NMR experiments for assignment of proteins

Tried and tested for the 600 Inova spectrometer (as of May 21st 2005)

2D Heteronuclear Experiments


3D Triple Resonance Experiments

(for relative sensitivity - click here)

3D Experiments Giving Dipole-Dipole/Chemical exchange information
including:
3D Time-Shared NOESY for optimal resolution


4D experiments

Back to group wisdom



Relaxation Experiments

Varian Setup

Heteronuclear NOE

For setup and analysis of T1 and T2 data from Varian







Click Here for 2D 1H-15N relaxation Experiments (bruker)

Back to group wisdom


NMRPipe Stuff

NMRPipe processing of Varian experiments


Back to group wisdom


Other Stuff


Mike's Translational diffusion analysis programme


Mike's scripts for converting NMRView formats to aria formats



Problems phasing in the indirect dimension?



Click for converting NMRPipe format to NMRView, SPARKY and XEASY


Click here for NMRVIEW stuff


Click here for pulse programmes


Other useful tips




2D Heteronuclear Experiments



2D 15N-1H HSQC with gradient selection and sensitivity enhancement

2D 15N-1H HSQC "fast" hsqc with gradient selection

2D 13C-1H HSQC 

2D 13C-1H HSQC Constant time



3D Triple Resonance Experiments


For smaller proteins use coherence selection: for larger proteins you may lose sensitivity, in which case do not use coherence selection.




Note: for a protein of < 15kDa all these experiments should give reasonable spectra. These 5 spectra represent the minimum number of experiments (~ 2-3 days) required to yield complete assignments (except aromatic ring atoms)


A number of other experiments need to be optimised. Ask Mike if you have a particular experiment you wish to run


Back to group wisdom



NMR experiments for dipolar information



Back to group wisdom


4D experiments



Back to group wisdom




1H-15N Relaxation Experiments

Varian




15N Heteronuclear NOE on Varian inova

Setup

It is best to acquire these in an interleaved manner by doing the following steps (anything in red must be typed into vnmr)


Bruker


NMRView info






Other tips

       Temp calibration

        DSS standard