Realtive sensitivty of triple resonance experiments
Experiment
Assignment
Comment
relative s/n [%]
HNCO
H(i), N(i), C'(i-1)
< 20KDa, if above use 2H labeling
100
HNCA
H(i), N(i), Ca(i)/ Ca(i-1)
< 20KDa, if above use 2H labeling 50/15
HN(CO)CA
H(i), N(i), Ca(i-1) < 20KDa, if above use 2H labeling 71
HN(CA)CO
H(i), N(i), C'(i) < 20KDa, if above use 2H labeling 13/4
CBCA(CO)NH
H(i), N(i), Ca(i-1),Cb(i-1) < 20KDa, if above use 2H labeling 13/9
HBHA(CO)NH
H(i), N(i), Ha(i-1),Hb(i-1) < 20KDa, if above use 2H labeling 13/9
HNCACB
H(i), N(i), Ca(i),Cb(i)/Ca(i-1),Cb(i-1) < 15KDa, if above use 2H labeling 4/1.7 a/b(i)
1.3/0.5 a/b(i-1)