1H-15N het NOE experiments

setting up

processing

Set up

Beware: conventional BRUKER format is not followed in this experiment
I acquire an fid with presat and without presat in a sequential manner to eliminate any environmental effects during the experiment.
The second fid is written to a second directory (defined by wr #1) in the pulseprogramme
you need to define this directory using a file defined by DSNAM
this file should look something like this:

noe 2 1 /opt/xwinnmr/ozzy new

this will write every other fid to this directory

set the carrier to center of the NH's for presat and on water for the rest of the experiment
this is defined by an F1list e.g.

        O 600.130000
        4900.27                ;amides
        2806.5                   ;water

presat is obtained from a 120 1H pulse (p9) @ pl3. Calculate this appropiatley
presat period last for 3 seconds if L0 is et to 600 which is nomally sufficient.
make sure d1 (recycle delay) is at least 3*T1. 5 seconds at least, longer for smaller proteins.

Processing with nmrPipe

I use a cshell script that allows you to process multiple datasets click here to see an example
Output is to a directory defined in the script ($outname)
Both nmrPipe and nmrview format files are produced. Alter as necessary.
Usage is simple if you are in the NOE directory

<script_name> <1st exp no.> <final exp no.>

Check the script or ask Mike for further details.