Common Name |
Sequence Name(s) |
|
---|---|---|
PRESAT - includes PRESAT, WET, shaped-pulse PRESAT, jump-return, watergate (soft, 3919, W5) |
|
|
Saturation Transfer Difference 1D |
|
|
watergate COSY |
|
|
watergate DQFCOSY |
|
|
watergate NOESY |
|
|
watergate ROESY |
|
|
watergate TOCSY |
|
|
wet NOESY |
|
|
wet ROESY |
|
|
"quiet" NOESY |
|
|
CPMG-NOESY |
|
|
SS-NOESY |
|
|
NOESY-C_chirp_purge_lek_v3a |
|
|
C and/or N filtered NOESY |
|
|
magic-angle DQFCOSY |
|
|
z-filtered DIPSI-TOCSY |
|
|
CLEANEX N15-HSQC |
|
|
Fast N15-HSQC |
|
|
Fast N15-HSQC with Homodecoupling |
|
|
Fast N15-IPAP-HSQC with Homodec. |
|
|
N15-HMQC |
|
|
N15-HMQCJ |
|
|
N15-HSQC |
|
|
N15-HSQC with Homodecoupling |
|
|
N15-HSQC(IPAP) |
|
|
CPMG-N15-HSQC |
|
|
watergate N15-HSQC |
|
|
N15-T1 (TROSY) |
|
|
N15-T2 (TROSY) |
|
|
N15-NOE (TROSY) |
|
|
N15-NOE |
|
|
CRT-CPMG for NH |
|
|
N15-TOCSYHSQC |
|
|
N15-HSQCTOCSY |
|
|
N15-NOESYHSQC |
|
|
N15-HSQCNOESY |
|
|
C13-HMQCNOESYHSQC(4D) |
|
|
N15-HMQCNOESYHSQC(4D) |
|
|
N15-HSQCNOESYHSQC(3D) |
|
|
N15-HSQCNOESYHSQC(4D) |
|
|
N15-HSQCTOCSYNOESYHSQC(4D) |
|
|
C13-NOESYHSQC |
|
|
C13-NOESYHSQC with SE |
|
|
C13-HSQCNOESY |
|
|
C13-TOCSYHSQC |
|
|
C13-HSQCTOCSY |
|
|
N15,C13-NOESYHSQC |
|
|
C13-HMQC |
|
|
C13-HMBC |
|
|
CT-C13-HMQC |
|
|
Fast 13C-HSQC |
|
|
C13-HSQC |
|
|
2H pw90 calib |
|
|
2H decoupling |
|
|
C(CO)NH (or C(CC-TOCSY-CO)N-NH ) |
|
|
H(CCO)NH (or H(CC-TOCSY-CO)N-NH ) |
|
|
CBCA(CO)NH |
|
|
CBCANH |
|
|
HCACO |
|
|
HCACON |
|
|
HCACOCANH |
|
|
HNCO |
|
|
HNCO_JNH |
|
|
HNCO_NOE |
|
|
HNCOCO |
|
|
HNHA |
|
|
HNHB |
|
|
HNN |
|
|
HNCN |
|
|
LR-JCH |
|
|
LR-JCC |
|
|
HN(CO)HB |
|
|
HNCA |
|
|
HCAN |
|
|
CT-HNCA |
|
|
HNCACB |
|
|
CT-HNCACB |
|
|
HN(CO)CA |
|
|
HN(CA)CO |
|
|
HN(COCA)CB |
|
|
C-CH-TOCSY |
|
|
HCCH-TOCSY |
|
|
HCCH-COSY |
|
|
DE-H(C)CH-TOCSY |
|
|
aromatic proton-beta carbon correlation |
|
|
3D C(a)-H(b)-H(a) correlation |
|
|
3D C(b),C(a)-CO-H(a) correlation |
|
|
3D HBHA(CO)NH |
|
|
Intraresidue-only 3D HNCA |
|
|
Intraresidue-only 3D HNCACB |
|
|
S3 N15-HSQC |
|
|
S3 ab-filtering for J(NH) |
|
|
NOESYHSQC 3D for heterodimers |
|
|
S3 for J(N-CO)/(HN-CO) doublets |
|
|
S3 J(NCa) in 1H-15N correlation |
|
|
3D 1J(HaCa) and 2J(N(i)H(i)) |
|
|
S3 2D J(CoCa) in a 1H-15N correlation |
|
|
S3 3D J(N-CO) in 1H-15N-13CO correlation |
|
|
S3 3D 1J(NCa), 2J(NCa), 2J(HNCa) & 3J(HNCa) |
|
|
S3 3D 1J(COCa) & 3J(HNCa) |
|
|
3D C(methyl-CT)-noesy-C(methyl-CT)-H(methyl) |
|
|
4D 13C,15N edited NOESY with TROSY |
|
|
4D 15N,15N edited NOESY with TROSY |
|
|
3D HNCA with TROSY (deuterated) |
|
|
3D HN(CA)CB with TROSY (deuterated) |
|
|
3D HN(CA)CO with TROSY (deuterated) |
|
|
4D HN(CA)CO with TROSY (deuterated) |
|
|
3D HNCO with TROSY (deuterated) |
|
|
3D HN(CO)CA_SEQ with TROSY (deuterated) |
|
|
4D HN(CO)CA_SEQ with TROSY (deuterated) |
|
|
4D HN(CO)CA_SIM with TROSY (deuterated) |
|
|
3D HN(CO)(CA)CB with TROSY (deuterated) |
|
|
References are given in manual files and |
||
Many sequences have a TROSY option, with gradient selection. |
||
Sequences marked "deuterated" should only be used on fully deuterated proteins (no proton decoupling used). |
Common Name |
Sequence Name(s) |
|
---|---|---|
N15-HSQC |
|
|
C(CO)NH (or C(CC-TOCSY-CO)N-NH) |
|
|
CBCA(CO)NH |
|
|
CBCANH |
|
|
H(CCO)NH (or H(CC-TOCSY-CON-NH) |
|
|
DE-H(C)CH-TOCSY |
|
|
HCCHTOCSY |
|
|
HNCA |
|
|
HN(CA)CO |
|
|
HNCACB |
|
|
HNCO |
|
|
HN(CO)CA |
|
|
These have been written to provide the same features as the above equivalents. |
Common Name |
Sequence Name(s) |
|
---|---|---|
1H PRESAT, WET, jump-return, watergate |
|
|
1H PRESAT-NOESY |
|
|
1H PRESAT-DQCOSY |
|
|
1H WET-ROESY |
|
|
1H WATERGATE-ROESY |
|
|
1H WATERGATE-NOESY |
|
|
1H WET-NOESY |
|
|
1H WET-TOCSY |
|
|
1H SS-NOESY |
|
|
1H 1-1 echo NOESY |
|
|
13C HSQC |
|
|
13C TROSY |
|
|
15N HSQC |
|
|
15N TROSY |
|
|
13C HMQC |
|
|
13C HMQC-TOCSY |
|
|
13C CT-HMQC |
|
|
15N HMQC |
|
|
15N WG-HSQC |
|
|
15N HSQC(long-range) |
|
|
15N CPMG-HSQC |
|
|
13C NOESY-HSQC |
|
|
15N NOESY-HSQC |
|
|
gd-HCCH-TOCSY |
|
|
DE-H(C)CH-TOCSY |
|
|
HCCH-COSY |
|
|
HCCH-RELAY |
|
|
CPMG-NOESY |
|
|
HCN |
|
|
HCP |
|
|
HP-COSY |
|
|
C-HNCCCH |
|
|
U-HNCCCH |
|
|
A-HNC-TOCSY-CH |
|
|
A-HCCH-TOCSY |
|
|
G-HNC-TOCSY-CH |
|
|
HNN-COSY |
|
|
All of these can be run at any field strength after just
calibrating the 90's for 1H, 13C and 15N. All power levels are
automatically set for proper excitation. 2H decoupling is often
available as an option by setting dm3='nyn'
(and setting
proper values for channel 4 decoupling parameters). Operation is
valid only for UNITYplus and UNITY INOVA.
All r.f. events including pulses, spinlocks and decoupling are
automatically created for all field strengths by
autocalibrate/autoupdate for all sequences. Power levels for each of
these are then set by the pulse sequence itself, not the operator,
based on the high-power pw
, pwC
and pwN
calibrations. Optional power limits are available if the global
parameter BPcryogenic=1
. In this case, decoupling power
levels are checked and waveforms made appropriately during the
autocalibrate/autoupdate processes. These power limits are displayed
in the "Decoupling" Tcl-dg panel (VNMR) and in the BioPack
"Options" page (VnmrJ) within the ghn_co
Acquire pages.
All experiments in which CH protons are the relevant originating magnetizations or are the detected magnetization can be skipped for the case of fully-deuterated proteins (for automatic calibration or automatic 2D).
Two alternative methods of using phase-ramped pulses are included.
These methods call Pbox for shape creation at the time of dps
and go
. No shapes are required in shapelib for this
method. These sequences have a "P" or "A"
appended to the normal names. The former must be compiled the
bionmr.h
include file, while the latter require the
Pbox_bio.h
and Pbox_psg.h
include files (C
pulse sequences only).
The "P" versions use new psg elements which are written
with protein 13C bandwidths pre-defined so that terms such as "ca",
"co" and "cacb" are used. See the file
BioPack.bionmr
in ~/vnmrsys/manual
or in
/vnmr/manual/
for details. The operation, parameters and
performance of these is at least as good as the standard BioPack
sequences. The only differences are that the phi7cal value will be
different, and that the dof
value is always set to the
carbonyl carbon frequency for all experiments (contributed by Boban
John, Varian, and Robin Bendall).
The "A" versions include a header section of the pulse
sequence in which all shapes are created and parameter values from
these shapes are obtained at go
time. This mode of
operation allows conversion of existing pulse sequences to BioPack
versions without modification of the underlying codes (contributed by
Eriks Kupce, Varian, Sept. 2002). See the file BioPack.Asequences
in ~/vnmrsys/manual
or in /vnmr/manual
for
details on this approach, and how to convert an existing non-BioPack
sequence to a BioPack version.
These sequences are compiled during the installation.